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SpeciesReactivity
All species

Form
Stds=0-100 ng/ml ; Sample=50 ul; Sensitivity= 1 ng/ml; 65 min assay; Quantitative; Competitive assay; 96 tests

Samples
Serum. Other fluids not optimized.

KitContents
1. Anti-Cortisol coated strip plate (96 wells), Cat. 3501 1 plate 2. Cortisol Saliva Std. A (0 ng/ml), 10 ml , Cat # 3502A 1 vial 3. Cortisol Saliva Std. B (1 ng/ml), 0.6 ml , Cat # 3502B 1 vial 4. Cortisol Saliva Std. C (3 ng/ml), 0.6 ml , Cat # 3502C 1 vial 5. Cortisol Saliva Std. D (10 ng/ml), 0.6 ml , Cat # 3502D 1 vial 6. Cortisol Saliva Std. E (30 ng/ml), 0.6 ml , Cat # 3502E 1 vial 7. Cortisol Saliva Std. F (100 ng/ml), 0.6 ml , Cat # 3502F 1 vial 8. Cortisol Saliva Control Serum, 0.6 ml; Cat # 3503 (refer to concn on the vial) 1 vial 9. Assay buffer, 15 ml; Cat # 3504 1 bottle 10. Cortisol-HRP Conjugate (50X), 300 ul Cat # 3505,Dilute 1:50 in assay buffer before use, 1 bottle 11. TMB substrate, 16 ml, Cat # 3506 1 bottle 12. Stop solution (1N H2SO4), 6 ml; Cat. # T-30 1 bottle 13. Wash buffer (10X), 50 ml, Dilute 1:10, WB-10 1 bottle 14. Instruction Manual, M-3500 1

SpCrossreactivity
The following compounds were tested for cross-reactivity with the Direct Cortisol Saliva ELISA kit with cortisol cross-reacting at 100%; Cortisol, 100%, Prednisolone (13%), Corticosterone, Deoxycorticosterone, Progesterone, Cortisone, Deoxycortisol, Pednisone (5-7%), Dexamethasone (1-2%). No cross reaction was detected with DHEAS and Tetrahydrocortisone. Human cortisol kit has been designed and tested for human samples. Another kit for human serum or plasma is available. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in many species, this kit should work in most species as long as the steroid concentration is within the range of this kit.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.